![]() ![]() Several critical problems that might occur in a zebrafish laboratory include blockage of water supply to individual/all tanks in the housing system, poor water quality, and leakage in pipes or reservoir of the circulating system. Figure 4 illustrates a female zebrafish's ovipositor to help researchers distinguish between a male and a female zebrafish. Higher magnification view of a fertilized and an unfertilized egg is shown in Figure 3B and 3C respectively. ![]() Unfertilized embryos are generally opaque and/or with ruptured cell(s) inside the chorion (black arrow) whilst fertilized embryos appears intact and growing to the next cell division state (for detailed reading of different stages of zebrafish embryos see 1). Figure 3A illustrates a microscopic view of fertilized and unfertilized embryos. Fertilized and unfertilized eggs are shown in Figure 3. This will help in understanding the difference between unfertilized and fertilized brine shrimps for proper feeding purposes. Figure 2 depicts a microscopic view of brine shrimps at 12X ( Figure 2A), a single brine shrimp at 90X ( Figure 2B), and an unfertilized brine shrimp egg at 90X ( Figure 2C). An illustration of a male zebrafish ( Figure 1A) and female zebrafish ( Figure 1B & 1C) is shown to help researchers distinguish between a male and a female zebrafish for breeding purpose. In addition, the following illustrations will help identify fertilized eggs, adult zebrafish, and their food. As a result of this protocol, researchers will be able to manage a zebrafish facility which will provide healthy conditions to the zebrafish. Several thousand zebrafish can be housed in a small laboratory. Zebrafish housing and maintenance is easier and cheaper than traditional rodent models. This protocol will be of immense help to researchers planning to establish a zebrafish laboratory, and also to graduate students who are intending to use zebrafish as an animal model. ![]() This process will help researchers to understand the natural behaviour and optimal conditions of zebrafish husbandry and hence troubleshoot experimental issues that originate from the fish husbandry conditions. This video illustrates the protocol for regular housing, feeding, breeding and raising of zebrafish larvae. Although literature is available for the maintenance of a zebrafish laboratory, a concise video protocol is lacking. Due to the spur of interest in zebrafish research, the need to establish and maintain a productive zebrafish housing facility is also increasing. The advantages of zebrafish in comparison to other common vertebrate models include high fecundity, low maintenance cost, transparent embryos, and rapid development. As a vertebrate, zebrafish share considerable genetic sequence similarity with humans and are being used as an animal model for various human disease conditions. Zebrafish are now gaining popularity in genetics, pharmacological and behavioural research. For up-to-date information on our current stock levels, we advise you to contact your local store directly.This protocol describes regular care and maintenance of a zebrafish laboratory. *Note that our store selection of fish may vary from time to time. PH: Slightly alkaline to neutral water pH between 6.5-7.5.ĭiet: The Zebra Danio is an omnivore and will eat a variety of foods, including flakes, pellets, and live or frozen foods such as brine shrimp and bloodworms. Temperature: Water temperature between 64-75☏ (18-24☌). Max Size: Typically grow to be around 2 inches (5 cm) in length. Its distinctive striped pattern and active swimming behavior make it a popular choice for aquarists of all levels. Overall, the Zebra Danio is a hardy and active fish that is great for beginner aquarists. They are hardy fish that can tolerate a wide range of water conditions, making them ideal for beginner aquarists. The aquarium should also be well-filtered and well-aerated to maintain good water quality. When keeping Zebra Danios in an aquarium, it is important to keep them in groups of at least six individuals. In the wild, Zebra Danios are found in shallow, slow-moving streams and ponds in India, Pakistan, Bangladesh, Nepal, and Bhutan. They are social fish that live in large schools and are known for their active swimming behavior. It typically grows to a maximum size of 2 inches (5 cm). The Zebra Danio has a small, slender body with a silver coloration and blue stripes that run the length of its body. It is known for its distinctive striped pattern and active swimming behavior. The Zebra Danio, also known as Danio rerio, is a popular freshwater fish that is commonly kept in home aquariums. ![]()
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